Co-transformation of bean callus using high-velocity microprojectiles- mediated DNA transfer
We have found that 50 mg/I kanamycin and 0.8 Mo1/1 mannitol concentration was sufficient to kill the control callus of bean (Phaseolus vulgaris L.) and differentiate transgenic from the non-transgenic cells.The GeneBooster particle delivery system was used for the bombardment of bean callus.The kanamycin resistance gene was used Special Tags as a s